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The funds were raised through donations from dog owners who accessed the team's on-site service during the event.
One of the team, Rita Johnson RVN, said: "After learning about the challenges facing veterinary teams in Ukraine, the Crufts volunteer vet team wanted to support this appeal to help them continue their vital work."
Mark Johnston, Vice President of the Prosalus Foundation, said: "We were deeply moved to receive this generous donation from the volunteer vet team at Crufts.
"We thank them for their concern for veterinary teams in Ukraine and the millions of animals caught up in the conflict."
He added: "We hope more vets in the UK will follow their example and help us to help our dedicated Ukrainian colleagues through these desperate times."
UK veterinary professionals can support the campaign by donating veterinary medicines, equipment and supplies, making a cash donation online via The ProSalus Foundation's website, or bidding in a silent auction of Ukrainian art.
www.prosalusfoundation.org