https://www.vetnurse.co.uk/f/clinical-discussions/19527/acth-stim-test Hi, I was wondering if anyone could explain, step by step, how an ACTH Stim Test is performed in house using Idexx machines. Also, what time schedules are reccomended between taking 1st blood, giving synathen (sic) and taking 2nd blood. thank so muchen-USTelligent Community 10https://www.vetnurse.co.uk/thread/136711?ContentTypeID=1Sun, 18 Nov 2012 17:53:31 GMT1a0763ec-3885-442c-853e-6cef656dfec5:a4ec7169-9a7a-416c-a902-b57c44ca93c4JaneRVN<p>Thats ok!! Most people dont realise yo can start as early as 30 mins post cortisol. I only know as it was 30 mins to get a blood sample from my elderly Peke (involving 4 members of staff...) so we were prepared!</p><div style="clear:both;"></div>https://www.vetnurse.co.uk/thread/136672?ContentTypeID=1Sat, 17 Nov 2012 09:48:38 GMT1a0763ec-3885-442c-853e-6cef656dfec5:71c61697-134c-4a38-9efc-a1fc4b13685eJaneRVN<p>It depends on the lab - some suggest min 30 mins between and max 60 mins between. I&#39;ve not heard as long as 90 mins - Idexx say 1 hour post cortisol and if with a difficult patient would allow 10&nbsp; mins over the hour!</p><div style="clear:both;"></div>https://www.vetnurse.co.uk/thread/136599?ContentTypeID=1Wed, 14 Nov 2012 22:20:55 GMT1a0763ec-3885-442c-853e-6cef656dfec5:e0de8a35-43e3-4d92-ad24-549bcfee5a59Steph Worsley<p>the instructions for running the sample should be in your handbook/in the cortisol snap tests you get from Idexx</p> <p>take first sample</p> <p>inject synacthen (IV straight away)</p> <p>take 2nd sample 1 hour later</p> <p>exactly the same protocol as sending the sample away to the external labs</p><div style="clear:both;"></div>