total protein using refractometer

Re: total protein using refractometer

Nick Shackleton — Sun, 27 May 2012 17:10:07 GMT

[quote user="hissycat"]

cheers. so I can snap a spun down capillary tube and use that?

[/quote]

not ideal as you can scratch the surface of the prism plus the H&S implications I've always used a pipette or insulin syringe

Re: total protein using refractometer

Steph Worsley — Sat, 26 May 2012 17:21:59 GMT

yep...sorry, snap a capillary tube just above the buffy coat and use the serum

Re: total protein using refractometer

hissycat — Sat, 26 May 2012 16:38:18 GMT

cheers. so I can snap a spun down capillary tube and use that?

Re: total protein using refractometer

Steph Worsley — Fri, 25 May 2012 18:28:09 GMT

that's ok, but it is only for effusions not serum total protein and is in g/dl whereas it is usually read as g/l in the UK

I am working this weekend so will try and sort the grah we have at work for people!

Re: total protein using refractometer

A-J — Fri, 25 May 2012 09:57:44 GMT

how about this

http://www.futurevows.com/doc/AdvancedMedicine/Abdominal,%20thoracic,and%20pericardial%20effusions.pdf

page 4 of the pdf but pg 93 of the article (table 2)

Re: total protein using refractometer

Sal the 1st — Fri, 25 May 2012 09:44:43 GMT

I am glad you cant find a conversion table on line - now I know it isnt just me, have been looking for one for ages

Re: total protein using refractometer

Steph Worsley — Thu, 24 May 2012 23:27:55 GMT

not all refractometers ave gm/dl we have an nd scale which is 1.340 etc and a conversion table for serum and exudate which I can't find online sorry!! I can try and remember to note down the main points so you can plot your own graph if you want

Re: total protein using refractometer

A-J — Thu, 24 May 2012 23:06:43 GMT

drop of serum onto glass, lid down and read gm/dl