https://www.vetnurse.co.uk/f/clinical-discussions/14790/urine-sample-please-back-me-up Please tell me i am correct in saying there is no point in doing a urine sample for microscopy if it is a day old? Having a daft moment (happening more and more latley! en-USTelligent Community 10https://www.vetnurse.co.uk/thread/119648?ContentTypeID=1Thu, 08 Sep 2011 10:05:07 GMT1a0763ec-3885-442c-853e-6cef656dfec5:72114801-a28e-4d05-9e05-3dd714fae9fbdaisy<p>latest research shows that to obtain accurate results, the urine&nbsp;shouldn&#39;t&nbsp;be over 3 hours old and stored at room&nbsp;temperature. the main discrepancy in urine older that 3 hours was the leukocyte count, it can in fact increase by 50%.&nbsp;</p><div style="clear:both;"></div>https://www.vetnurse.co.uk/thread/119508?ContentTypeID=1Sun, 04 Sep 2011 22:57:56 GMT1a0763ec-3885-442c-853e-6cef656dfec5:7723b9ce-78c7-479c-aaf7-de017a808283Sal the 1st<p>it depends on pH , temperature, crystals&nbsp;and whether of not the sample is capped.</p> <p>Samples that are warm and have opportunity to evaporate then yes you will get crystals growing but if there is a lot of bacteria in the urine which are allowed to multiply this may significantly affect the pH and allow crystals to dissolve.</p> <p>Try it for yourself next time you get a&nbsp; good urine sample with struvite in - look at some fresh and see how crisp the crystal edges are, do a pH and &nbsp;keep some at room temperature and look at it a day later, do a comparitive pH- see if it has changed&nbsp;and see how many more crystals you have or if you have a significant increase in bacteria and crystals with rounded edges which are dissolving</p> <p>Hope that makes sense - makes perfect sense to me but I am not always the best at describing stuff</p><div style="clear:both;"></div>https://www.vetnurse.co.uk/thread/119504?ContentTypeID=1Sun, 04 Sep 2011 21:16:10 GMT1a0763ec-3885-442c-853e-6cef656dfec5:753556d1-35d7-408b-913c-273701f3af0fNuttyNu<p>I was always told in practice by other nurses that they dissolved in time. We haven&#39;t covered lab at college yet so I may be wrong!</p> <p>Many apologies if I am!</p><div style="clear:both;"></div>https://www.vetnurse.co.uk/thread/119501?ContentTypeID=1Sun, 04 Sep 2011 20:20:00 GMT1a0763ec-3885-442c-853e-6cef656dfec5:98e1d184-ec30-4f0e-952a-3ae64d8039bbSusan Jackson<p>Ok now thats confused me. I thought crystals formed over time. So in an old sample your likely to see crystals that were not there originally when the sample was produced. Now NuttyNu saying they dissolve over time? Is that just a mistake or do thery form then dissolve?</p><div style="clear:both;"></div>https://www.vetnurse.co.uk/thread/119498?ContentTypeID=1Sun, 04 Sep 2011 19:36:40 GMT1a0763ec-3885-442c-853e-6cef656dfec5:59fc4fa4-9d5b-4598-b43c-03f92aeee4deNuttyNu<p>I would say no as well. Crystals are known to dissolve over time as well so if you are looking for crystals, it wouldn&#39;t be accurate results.</p> <p>&nbsp;</p><div style="clear:both;"></div>https://www.vetnurse.co.uk/thread/119092?ContentTypeID=1Sat, 27 Aug 2011 12:22:12 GMT1a0763ec-3885-442c-853e-6cef656dfec5:fa26f9be-4786-43ce-a63b-0e663915dcc8PaperTiger<p>Thanks guys, sometimes you just get days where you can&#39;t even remember how to spell your name! </p><div style="clear:both;"></div>https://www.vetnurse.co.uk/thread/119090?ContentTypeID=1Sat, 27 Aug 2011 11:50:29 GMT1a0763ec-3885-442c-853e-6cef656dfec5:8674776a-b100-4b51-9170-0a261b7f97e7Nicola Smith<p>No. crystals may form in urine left to stand - needs to be fresh.</p><div style="clear:both;"></div>https://www.vetnurse.co.uk/thread/119085?ContentTypeID=1Sat, 27 Aug 2011 11:30:45 GMT1a0763ec-3885-442c-853e-6cef656dfec5:9b35b611-a02d-4c41-93e0-049bd18a7acfStuart McQueen RVN MBVNA<p>I wouldn&#39;t bother.... no!</p><div style="clear:both;"></div>